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Am. J. Respir. Crit. Care Med., Volume 162, Number 3, September 2000, 1175-1177

Exhaled 8-Isoprostane as an In Vivo Biomarker of Lung Oxidative Stress in Patients with COPD and Healthy Smokers

PAOLO MONTUSCHI, JOHN V. COLLINS, GIOVANNI CIABATTONI, NICOLA LAZZERI, MASSIMO CORRADI, SERGEI A. KHARITONOV, and PETER J. BARNES

Imperial College School of Medicine at the National Heart and Lung Institute, Department of Thoracic Medicine, London, United Kingdom; and Institute of Pharmacology, School of Medicine, Catholic University of the Sacred Heart, Rome, Italy

Most of the studies linking chronic obstructive pulmonary disease (COPD) with oxidative stress are in vitro, using invasive techniques, or measuring systemic oxidative stress. The aim of this study was to quantify oxidative stress in the lungs in patients with COPD and in healthy smokers, as reflected by 8-isoprostane concentrations in breath condensate. This is a noninvasive method to collect airway secretions. 8-Isoprostane is a prostaglandin-F2alpha isomer that is formed in vivo by free radical-catalyzed peroxidation of arachidonic acid. We also studied the acute effect of smoking on exhaled 8-isoprostane in healthy smokers. Exhaled 8-isoprostane was measured by a specific enzyme immunoassay in 10 healthy nonsmokers and 12 smokers, 25 COPD ex-smokers, and 15 COPD current smokers. 8-Isoprostane concentrations were similar in COPD ex-smokers (40 ± 3.1 pg/ml) and current smokers (45 ± 3.6 pg/ ml) and were increased about 1.8-fold compared with healthy smokers (24 ± 2.6 pg/ml, p < 0.001), who had 2.2-fold higher 8-isoprostane than healthy nonsmokers (10.8 ± 0.8 pg/ml, p < 0.05). Smoking caused an acute increase in exhaled 8-isoprostane by about 50%. Our study shows that free radical production is increased in patients with COPD and that smoking causes an acute increase in oxidative stress.




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