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Am. J. Respir. Crit. Care Med., Volume 157, Number 2, February 1998, 654-657

Surfactant Protein A Is Decreased in a Rat Model of Congenital Diaphragmatic Hernia

MOHAN R. MYSORE, LINDA R. MARGRAF, MARINA A. JARAMILLO, DAVID R. BREED, VICKEY L. CHAU, MAURICIO ARÉVALO, and FERNANDO R. MOYA

Departments of Pediatrics, Pathology, and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, Texas

We hypothesized that the expression of surfactant protein A (SP-A) would be altered in developing lungs from rat fetuses with congenital diaphragmatic hernia (CDH) induced by maternal ingestion of 2,4-dichlorophenyl-p-nitrophenyl ether (Nitrofen) on Day 9 of gestation. We compared our findings in fetuses exposed to Nitrofen with a CDH with those in Nitrofen-exposed fetuses without a CDH, and control fetuses whose mothers received olive oil only, the vehicle for Nitrofen. In late gestation, immunocytochemistry using a polyclonal rabbit antihuman SP-A antibody revealed decreased amounts of this protein in lungs from fetuses with CDH. Using immunoblotting, the relative amount of SP-A on Day 21 of gestation was also decreased in lung tissue from fetuses with CDH compared with the other groups. Abnormalities of mRNA for SP-A were observed in both groups of Nitrofen- exposed fetuses compared with control rats. These findings suggest that there is decreased expression of SP-A in rat fetuses with CDH secondary to Nitrofen exposure.




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