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Am. J. Respir. Crit. Care Med., Volume 156, Number 5, November 1997, 1593-1600

TCR Vbeta Families in T Cell Clones from Sarcoid Lung Parenchyma, BAL, and Blood

GERNOT ZISSEL, IRINA BÄUMER, BERNHARD FLEISCHER, MAX SCHLAAK, and JOACHIM MÜLLER-QUERNHEIM

Research Center Borstel, Medical Hospital, Borstel, and Bernhard-Nocht-Institute, Institute for Tropical Medicine, Hamburg, Germany

The TCR repertoire and the CD4/CD8 ratio of clones from peripheral blood (PB), transbronchial biopsies (TBB), and bronchoalveolar lavage (BAL) of 16 sarcoid patients was analyzed by staining the clones with monoclonal antibodies against nine Vbeta -families. We observed a striking increase in the CD4/ CD8 ratio of the clones from BAL; whereas the CD4/CD8 ratio of the clones from PB was in the normal range. The CD4/CD8 ratio of the TBB-clones had also increased, but this increase did not reach the level of that of the BAL clones. The most prominent changes in the Vbeta percentages could be detected in the CD4+ subpopulation of the BAL-clones. The most abundant Vbeta families were Vbeta 5 in PB and BAL (11.8 and 28.6%, respectively) and Vbeta 6 in the TBB (12.4%). A similar compartmentalized Vbeta usage could be demonstrated in one patient with tuberculosis and one patient with HP. The increase in Vbeta 5, Vbeta 8, Vbeta 12, Vbeta 13.3, and Vbeta 19 in the BAL and the increase of Vbeta 5, Vbeta 6, Vbeta 13.3, and Vbeta 19 in the TBB suggest an antigen-driven activation of the T cells in both compartments. Differences in the Vbeta percentages between BAL and TBB and the lower CD4/CD8 ratio in the TBB, however, demonstrate a relative independence of the two compartments.




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